107760 HPLC of glutathione conjugates I. Ion pair reversed phase chromatography and UV detection
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HPLC of glutathione conjugates I. Ion pair reversed phase chromatography and UV detection
Summary
Type: HPLCApplication #: 107760
Catalog#: 720014.40 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4x250mm
Detection: UV, 270nm
Conditions: eluent: water - methanol (65:35) containing 10mmol/l tetrabutylammonium hydrogen sulphate flow rate 0.7ml/min
Authors: Tsikas D
Source: J Anal Chem 343 (1992) 326 - 329
Details
Legend: Fig. 3. Chromatogram of a standard mixture of glutathionyl conjugate, cysteinyl conjugate, mercapturic acid of p-nitrobenzyl chloride (PNBC), and PNBC (500µmol/l each).