107740 Isocratic separation of PTH amino acids by HPLC

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Isocratic separation of PTH amino acids by HPLC

Summary

Type: HPLC
Application #: 107740
Catalog#: 720014.40 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4x250mm
Detection: UV, 254nm
Conditions: eluent: 32% acetonitrile - tetrahydrofuran (9:1) + 68% tetrahydrofuran - water (50:950) containing 0.3 ml/l acetic acid, adjusted to pH 5.15 with 1N NaOH
flow rate 1.5ml/min
temperature 60°C
pressure 2500psi
Authors: Kim SM
Source: Chemie Grünenthal Stolberg Germany


Details


Legend: Separation of PTH amino acids (~ 0.5nmol) 1. aspartic acid 2. carboxymethyl cysteine 3. glutamic acid 4. asparagine 5. glutamine 6. serine 7. threonine 8. glycine 9. histidine 10. arginine