107720 Indirect UV detection of hydrophilic ionized compounds in reversed phase LC by use of a UV-absorbing ion of the same charge

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Indirect UV detection of hydrophilic ionized compounds in reversed phase LC by use of a UV-absorbing ion of the same charge

Summary

Type: HPLC
Application #: 107720
Catalog#: 720002.40 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4x125mm
Detection: UV, 254nm
Conditions: temperature 35°C
eluent: 2·10-4M nicotinamide, 10-3M Na n-octyl sulphate in 0.05M phosphoric acid
flow rate 0.5ml/min
Authors: Crommen J
Source: J Pharm Biomed Anal 2 (1984) 241 - 253


Details


Legend: Fig. 1. Indirect UV detection of hydrophilic amino acids (0.5 - 1.5µg), 1. glycine, 2. aspartic acid, 3. threonine, 4. glutamic acid, 5. alanine, 6. cysteine, 7. proline, 8. a-aminobutyric acid, sys