107670 Highly sensitive assay for g-glutamyltranspeptidase activity by HPLC with electrochemical detection

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Highly sensitive assay for g-glutamyltranspeptidase activity by HPLC with electrochemical detection

Summary

Type: HPLC
Application #: 107670
Catalog#: 720018.40 - MN HPLC Column, Nucleosil C18, 100A, 7um, 4x250mm
Detection: electrochemical, potential set to +0.55V versus Ag/AgCl
Conditions: eluent: 0.1M Na phosphate buffer pH 3.5 containing 10μM EDTA - methanol (94:6, v/v)
flow rate 0.9ml/min
Authors: Kiuchi K
Source: J Chromatogr 357 (1986) 191 - 198


Details


Legend: Fig. 1. HPLC of A) mixture of standards in 0.01M hydrochloric acid (12.5pmol each of 1. dopa, 2. a-methyldopa (Mdopa) and 3. g-Glu-dopa), B) eluate from an aluminium oxide column, when 12.5pmol each