107640 Measurement of reduced and oxidized glutathione in cultures of adult rat hepatocytes

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Measurement of reduced and oxidized glutathione in cultures of adult rat hepatocytes

Summary

Type: HPLC
Application #: 107640
Detection: UV, 365nm
Conditions: eluent A: methanol - water (4:1, v/v), eluent B: 200ml of a mixture of 272g Na acetate trihydrate, 122ml water, 378ml glacial acetic acid plus 800ml eluent A; 5min 25% B, linear gradient to 95% B over 8min, hold 4min
flow rate 2.0ml/min
Authors: Mertens K
Source: J Chromatogr 565 (1991) 149 - 157


Details


Legend: Fig. 1A. Separation of a standard mixture of GSH and GSSG (60nmol/ml) as 2,4-dinitrophenyl derivatives.