107630 Determination of hippuric acid and methylhippuric acid in urine by HPLC

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Determination of hippuric acid and methylhippuric acid in urine by HPLC

Summary

Type: HPLC
Application #: 107630
Catalog#: 720149.40 - MN HPLC Column, Nucleosil C18, 120A, 3um, 4x100mm
Detection: UV, 225nm
Conditions: eluent: Na phosphate buffer - methanol (85:15, v/v)
flow rate 0.6ml/min
pressure 248atm
Authors: Müller J
Source: Inst f Arbeits- und Sozial-Medizin Univ Erlangen-N


Details


Legend: Chromatogram of hippuric acid (HA) and methylhippuric acid (MHA) isomers in urine, retention times: 1. HA, 6.04min, 2. o-MHA, 8.13min, 3. p-MHA, 15.06min, 4. m-MHA, 15.54min. also see K. Goßler,