107620 Use of gradient elution for rapid selection of isocratic conditions in reversed phase HPLC

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Use of gradient elution for rapid selection of isocratic conditions in reversed phase HPLC

Summary

Type: HPLC
Application #: 107620
Catalog#: 720023.46 - MN HPLC Column, Nucleosil C18, 100A, 10um, 4.6x250mm
Detection: UV, 254nm
Conditions: eluent: methanol - water, acetonitrile - water and tetrahydrofuran - water as indicated, 1mM perfluorooctanoic acid added in B, C and D
flow rate 1.5ml/min
Authors: Schoenmakers, P.J. et al.
Source: J. Chromatogr. 205 (1981) 13–30


Details


Legend: Fig. 14. Isocratic elution of PTH amino acids, 1. glycine, 2. alanine, 3. arginine, 4. glutamic acid, 5. methionine, 6. phenylalanine, 7. isoleucine, 8. leucine.