107580 HPLC determination of desmosine and isodesmosine after phenylisothiocyanate derivatization

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HPLC determination of desmosine and isodesmosine after phenylisothiocyanate derivatization

Summary

Type: HPLC
Application #: 107580
Catalog#: 720041.40 - MN HPLC Column, Nucleosil C18, 120A, 5um, 4x250mm
Detection: UV, 254nm
Conditions: temperature 32°C
eluent: 10mM tetrabutylammonium phosphate in 80mM phosphate buffer pH 6.5 - methanol - acetonitrile (50:16:34)
flow rate 1ml/min
Authors: Salomoni M
Source: J Chromatogr 572 (1991) 312 - 316


Details


Legend: Fig. 2. Chromatograms of a) an elastin standard (1µg/ml) and b) a rat aorta sample.