107570 HPLC system for development of new arginine protecting groups

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HPLC system for development of new arginine protecting groups

Summary

Type: HPLC
Application #: 107570
Catalog#: 720014.40 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4x250mm
Detection: post column reaction with 1N NaOH, 0.3ml/min, 2.5g/l 9,10-phenanthrenequinone in ethanol, 0.5ml/min and 5% H2SO4, 0.3ml/min, temperature of the reaction coil 95 °C
Conditions: eluent A: acetonitrile - water, 0.06% TFA (20:80), eluent B: acetonitrile - water, 0.5% TFA (90:10), linear gradient from A to B in 15μin
flow rate 1 ml/min
Authors: Voelter W
Source: Chromatographia 30 (1990) 719 - 723


Details


Legend: Fig. 6 and 7. Chromatogram of Z-Arg(Mtr)-OH after treatment with trifluoroacetic acid (TFA) / thioanisole (4:1) for 30min. fig. 7. fluorescence, excitation 315nm, emission 395nm see also G. Grübl