107560 A chemiluminescence detector for trace determination of fluorescent compounds

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A chemiluminescence detector for trace determination of fluorescent compounds

Summary

Type: HPLC
Application #: 107560
Catalog#: 720014.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x250mm
Detection: reagents for chemical excitation: 5mM bis(2,4,6-trichlorophenoxy)oxalate (TCPO) in ethyl acetate, flow rate 0.5ml/min, and 30% (v/v) H2O2, diluted with acetone to 0.5M; detection: chemiluminescence
Conditions: eluent: acetonitrile - 0.05M TRIS / HCl buffer, pH 7.7 (35:65, v/v)
flow rate 1ml/min
Authors: Mellbin G
Source: J Liquid Chromatogr 6 (1983) 1603 - 1616


Details


Legend: Chromatogram of amino acid dansyl derivatives. Sample concentrations: dansyl-L-methionine (MET) 2.07·10-10mol/l, dansyl-L-glutamic acid (GLU) 1.73·10-10mol/l, dansyl-L-a-alanine (ALA) 2.37·10-10mo