107530 Isolation of high-molecular-weight nucleic acids for copy number analysis using HPLC

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Isolation of high-molecular-weight nucleic acids for copy number analysis using HPLC

Summary

Type: HPLC
Application #: 107530
Catalog#: 736601 - MN HPLC Column (Valco type), Nucleogen DEAE, 4000A, 7um, 6x125mm
Detection: UV, 260nm
Conditions: buffer A: 5M urea, 20 mM K phosphate pH 6.9, buffer B: A + 1.5M KCl, 15% B to 95% B in 50min
flow rate 1ml/min
Authors: Coppella S
Source: J Chromatogr 402 (1987) 189 - 199


Details


Legend: Fig. 2. Comparison of elution of recombinant yeast cell lysate with standards, A) recombinant yeast AB103.1 pYaEGF-25 lysate, not boiled, 1. unretained, 2. tRNA, 3. rRNA, 4. plasmid/chromosomal DNA,