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HPLC in rapid DNA sequencing of kilobase pairs of DNA
Summary
Type: HPLCApplication #: 107520
Catalog#: 736601 - MN HPLC Column (Valco type), Nucleogen DEAE, 4000A, 7um, 6x125mm
Detection: UV, 256nm
Conditions: buffer A: 30mM K phosphate pH 6.5, 5M urea, buffer B: A + 2M KCl, 30% B to 55% B in 25min
flow rate 1ml/min
Authors: Dornburg R
Source: LC-GC 6 (1988) 254 - 259
Details
Legend: Fig. 1. Purification of a 3.2kbp DNA fragment after IEC elution from a 1% agarose gel.
also see Dornburg, R.; Kruppa, J.; Földi, P.; GIT 29 (1985) 505 - 515