107500 Separation of different tRNA molecules

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Separation of different tRNA molecules

Summary

Type: HPLC
Application #: 107500
Catalog#: 736601 - MN HPLC Column (Valco type), Nucleogen DEAE, 4000A, 7um, 6x125mm
Detection: UV, 280 nm (0.16 AUFS)
Conditions: eluents: buffer A = 20 mM sodium phosphate, pH 6.8, 10% MeOH, 200 mM NaCl; buffer B = 20 mM sodium phosphate, pH 6.8, 10% MeOH, 1000 mM NaCl; gradient: 35 - 50 % B in 30min
flow rate 1 ml/min
Authors: Macherey-Nagel
Source: LC department


Details


Legend: tRNA mixture, 1. tRNA Asp, 2. tRNA Gly, 3. unknown tRNA species, 4. unknown tRNA species, injection volume 2 µl, 1.9 µg/µl.