107490 Viroid research with biochemical, molecular biological and biophysical methods

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Viroid research with biochemical, molecular biological and biophysical methods

Summary

Type: HPLC
Application #: 107490
Catalog#: 736598 - MN HPLC Column (Valco type), Nucleogen DEAE, 60A, 7um, 10x125mm
Detection: UV, 260nm
Conditions: eluent A: 250mM KCl, 20mM phosphate buffer pH 6.6, 5M urea, eluent B: 1M KCl, 20mM phosphate buffer pH 6.6, 5M urea; linear gradient: 100% A to 100% B as indicated
flow rate 1ml/min
pressure 36bar
Authors: Riesner, D.
Source: BioEngineering 1 (1988), 42–48


Details


Legend: Fig. 1. Preparative separation of a crude RNA extract of viroid (PSTV) infected tomato plants.

also see M. Colpan and D. Riesner, J. Chromatogr. 296 (1984) 339 - 353.