107470 HPLC of DNA restriction fragments

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HPLC of DNA restriction fragments

Summary

Type: HPLC
Application #: 107470
Catalog#: 736601 - MN HPLC Column (Valco type), Nucleogen DEAE, 4000A, 7um, 6x125mm
Detection: Fig. 6: UV, 260nm Fig. 9.: UV, 260 nm Fig 7: UV, 260 nm Fig 8: UV, 260nm
Conditions: Fig. 6: eluent: linear gradient 700 - 1200mM KCl in 100min (5mM/min) in 6M urea - 30mM Na phosphate pH 6.0, flow rate 1ml/min; pressure 68bar; temperature 23°C

Fig. 9.: eluent: 500 - 1200mM KCl in 70min (10mM/min) in 6M urea - 25mM K phosphate pH 6.6, flow rate 1ml/min; pressure 48bar; temperature 24°C

Fig 7: eluent A: 20mM phosphate buffer pH 6.7, 6M urea, eluent B: buffer A + 1.2mM KCl, gradient from 500 to 1200mM KCl in 70min (a) and 350min (b), respectively, flow rate 2ml/min

Fig 8: eluent A: 20mM phosphate buffer pH 6.7, 6M urea, eluent B: buffer A + 1.2M KCl, gradient from 840 to 1200mM KCl in 70min (a) and 360min (b), respectively, flow rate a) 2ml/min, b) 2.25ml/min
Authors: Hecker R
Source: J Chromatogr 326 (1985) 251 - 261


Details


Legend: Fig. 6. HPLC of a set of restriction fragments from plasmid pBR 322 cleaved with restriction enzyme HinfI, total amount of DNA 10µg, lengths of restriction fragments: 1. 75 bp, 2. 154bp, 3. 220/221bp