107450 Fractionation of DNA restriction fragments with ion exchangers for HPLC

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Fractionation of DNA restriction fragments with ion exchangers for HPLC

Summary

Type: HPLC
Application #: 107450
Catalog#: 736598 - MN HPLC Column (Valco type), Nucleogen DEAE, 60A, 7um, 10x125mm
Detection: UV, 254nm
Conditions: Fig. 2: eluent: TE buffer pH 6.5, gradient from 0.3 to 1.25M KCl/165ml, flow rate 0.3ml/min; temperature 23°C

Fig. 3: eluent: 20mM K phosphate, 1mM EDTA, 6 M urea, pH 6.3, gradient from 0.225 to 1.2M KCl / 195ml, flow rate 0.5ml/min
Authors: Müller W
Source: Eur J Biochem 155 (1986) 203 - 212


Details


Legend: Fig. 1. Separation of DNA restriction fragments. Sample: pDS 1 - HaeIII digest, 14.4A260.

Fig. 2. Separation of DNA restriction fragments. Sample: pDS 1 - HaeIII digest, 10A260 + 1 A lineariz