107440 Analytical HPLC of a set of DNA restriction fragments from plasmid pBR 322

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Analytical HPLC of a set of DNA restriction fragments from plasmid pBR 322

Summary

Type: HPLC
Application #: 107440
Catalog#: 736601 - MN HPLC Column (Valco type), Nucleogen DEAE, 4000A, 7um, 6x125mm
Detection: UV, 260nm
Conditions: eluent A 0.5M NaCl, 6M urea, 30mM Na phosphate pH 6.0, eluent B 1.5M NaCl, 6M urea, 30mM Na phosphate pH 6.0, gradient 600mM NaCl to 1500mM NaCl in 180min, flow rate 1ml/min
Authors: Macherey-Nagel
Source: LC department


Details


Legend: Chromatogram of a set of restriction fragments from plasmid pBR 322, cleaved with HinfI.