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Separation of a deoxyoligonucleotide 18mer
Summary
Type: HPLCApplication #: 107410
Detection: UV 260 nm
Conditions: Buffer A: 40% acetonitrile, 0.02 M NaAc pH 5.5
Buffer B: buffer A + 0.7 M KCl
Gradient: 0–50% B in 120 min
50 – 100% B in 250 min
Flow rate: 3 ml/min, 40 bar
ambient temperature
Authors: Werntges H
Source: Diplomarbeit University of Düsseldorf
Details
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