107390 Separation of sequence isomeric pyrimidine oligodeoxynucleotides using reversed phase HPLC

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Separation of sequence isomeric pyrimidine oligodeoxynucleotides using reversed phase HPLC

Summary

Type: HPLC
Application #: 107390
Catalog#: 720018.46 - MN HPLC Column, Nucleosil C18, 100A, 7um, 4.6x250mm
Detection:
Conditions: eluent A: 0.1M ammonium acetate, eluent B: methanol - water (Fig. 5: 60:40 / Fig. 8: 73:27), A:B = 66:34
flow rate 1ml/min
Authors: Schott H
Source: J Chromatogr 280 (1983) 297 - 311


Details


Legend: Fig. 5. Separation of 0.9A260 units (dC3, dT), 1. (dC-dC-dC-dT) and
(dC-dC-dT-dC), 2. (dC-dT-dC-dC), 3. (dT-dC-dC-dC).

Fig. 8. Separation of 0.8A260 units (dC2, dT3), 1. impurities, 2.