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Analysis of oligonucleotides using coupled HPLC - electrospray MS
Summary
Type: HPLCApplication #: 107370
Detection: UV detection at 260 nm
Conditions: Fig. 3: eluent A: 10 mM ammonium acetate / 10% acetonitrile pH 8.3, eluent B: 10 mM ammonium acetate / 30% acetonitrile pH 8.3, gradient 0 - 100% B in 20min; flow rate 200 μl/min
Fig. 5: eluent A: 10 mM ammonium acetate pH 8.1, eluent B: 10 mM ammonium acetate / 10% acetonitrile pH 8.1, gradient 0 - 100% B in 20 min; flow rate and detection as above
Authors: Bleicher K
Source: Chromatographia 39 (1994) 405 - 408
Details
Legend: Fig. 3. Comparison of UV and TIC chromatograms of separation of three '5-