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Coupling of proteolytic quenching and HPLC to enzyme reactions
Summary
Type: HPLCApplication #: 107220
Detection: UV, 254nm
Conditions: eluent: linear gradient (dotted line) from 0.1 to 0.6M ammonium acetate solution pH 5.5
flow rate 1.0ml/min
Authors: Guasch A
Source: J Chromatogr 473 (1989) 281 - 286
Details
Legend: Fig. 1. Chromatography of the components of a reaction mixture (40mM C>p, 0.6 µM RNase A) incubated for 5min, stopped by addition of H3PO4 - HCl and then pepsin.