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Detection and separation of intracellular 1-
Summary
Type: HPLCApplication #: 107010
Catalog#: 720041.46 - MN HPLC Column, Nucleosil C18, 120A, 5um, 4.6x250mm
Detection: UV, 280nm
Conditions: eluent: aqueous solution with 0.1M KH2PO4, 0.005M tetrabutylammonium phosphate and 0.5% acetonitrile, adjusted to pH 2.7 with H3PO4
flow rate 0.8ml/min
1.6ml/min for backflush after 16min
temperature 40°C
Authors: Schleyer, E. et al.
Source: J. Chromatogr. 497 (1989) 109–120
Details
Legend: Fig. 1. HPLC separation of a standard mixture of CTP, Ara-CTP and dCTP (10µl injection, 10µg/ml Ara-CTP).