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HPLC separation of anthocyanins of Sambucus nigra L.
Summary
Type: HPLCApplication #: 106960
Catalog#: 720120.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x150mm
Detection: UV, 340nm
Conditions: eluent: tetrahydrofuran in 0.05M phosphoric acid pH 1.8, 1% - 40% THF in 15min
flow rate 1.2ml/min
Authors: Brønnum-Hansen, K. et al.
Source: J. Chromatogr. 262 (1983) 385–392
Details
Legend: Fig. 3. Chromatogram of raw extract after pH adjustment to pH 4.5 and heating. Flavylium cations: 1. cyanidin-3-sambubioside-5-glucoside, 2. cyanidin-3-glucoside-5-glucoside, 3. cyanidin-3-sambubios