106320 Determination of thyroxine (T4) in serum with HPLC

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Determination of thyroxine (T4) in serum with HPLC

Summary

Type: HPLC
Application #: 106320
Catalog#: 720150.46 - MN HPLC Column, Nucleosil C18, 100A, 3um, 4.6x125mm
Detection: fluorescence, excitation 338nm, emission 530nm
Conditions: eluent: gradient from 60% acetonitrile in 0.1% aqueous phosphoric acid to 10% acetonitrile as shown in upper trace
flow rate 1ml/min
Authors: Finke J
Source: Z Anal Chem 324 (1986) 318 - 319


Details


Legend: Fig. 1. Lower trace: chromatogram of hormone free serum; middle trace: chromatogram of patient serum after addition of T3 (9.9µg T3/dl and 6.3µg T4/dl); upper trace: shape of the acetonitrile gradie