106310 Determination of tetraiodothyronine in serum by HPLC and electrochemical detection

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Determination of tetraiodothyronine in serum by HPLC and electrochemical detection

Summary

Type: HPLC
Application #: 106310
Catalog#: 720018.40 - MN HPLC Column, Nucleosil C18, 100A, 7um, 4x250mm
Detection: Fig. 1. electrochemical, 800mV, 1nA sensitivity Fig. 2: electrochemical, 800mV, 5nA sensitivity
Conditions: Fig. 1. eluent: 1% H3PO4 - methanol (40:60, v/v), flow rate 0.8ml/min
Fig. 2: eluent: 0.1% H3PO4 - acetonitrile (70:30, v/v), flow rate 1.5ml/min
Authors: Besenfelder E
Source: HRC 7 (1984) 566 - 569


Details


Legend: Fig. 1. Chromatogram of A) T3 and T4, 5ng each, B) serum spiked with 50ng/ml T3 before cleanup, peaks: 1. T3, 2. T4. Fig. 2. Chromatogram of A) T3 and T4, 16 and 8ng, respectively, B) serum spiked