106290 Improved method for the determination of indole-3-acetic acid in plant tissue

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Improved method for the determination of indole-3-acetic acid in plant tissue

Summary

Type: HPLC
Application #: 106290
Detection: UV, 254nm
Conditions: eluent: 20min gradient from 0.1N acetic acid pH 2.8 to 0.1N acetic acid in 95% aqueous ethanol pH 4.2
flow rate 2.5ml/min
Authors: Kling GJ
Source: J Chromatogr 407 (1987) 377 - 383


Details


Legend: Fig. 1. Chromatogram showing UV absorbance of 1.0µg zeatin (A), 1.0µg zeatin-riboside (B), 2.0µg IAA (C) and 1.0µg abscisic acid (D) standards eluted from a two-column preparative HPLC system.