105860 Resolution of the stereoisomers of leucovorin and 5-methyltetrahydrofolate by chiral HPLC

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Resolution of the stereoisomers of leucovorin and 5-methyltetrahydrofolate by chiral HPLC

Summary

Type: HPLC
Application #: 105860
Catalog#: 720046.40 - MN HPLC Column, Resolvosil BSA-7, 300A, 7um, 4x150mm
Detection: UV, 282nm
Conditions: eluent: 5mM Na phosphate buffer pH 7.4
flow rate 1.0ml/min
Authors: Choi, K.E. et al.
Source: Anal. Biochem. 168 (1988) 398–404


Details


Legend: Fig. 2. Chromatogram of the separation of the two stereoisomers of commercially available leucovorin, 10µl of a 1mg/ml solution of [6R,S]-LV. Biologically active [6S]-isomer: first eluted peak (RT =