105830 Quantification of the enantiomers of ofloxacin in biological fluids by HPLC

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Quantification of the enantiomers of ofloxacin in biological fluids by HPLC

Summary

Type: HPLC
Application #: 105830
Catalog#: 720046.40 - MN HPLC Column, Resolvosil BSA-7, 300A, 7um, 4x150mm
Detection: Fig. 2: fluorescence, excitation 298nm, emission 458nm Fig. 3: detection as in fig. 2.
Conditions: Fig. 2: eluent: 0.2M phosphate buffer pH 8.0, 3% propan-2-ol, flow rate 1.0ml/min
Fig. 3: eluent: 0.2M phosphoric acid, adjusted to pH 1.85 with tetraethylammonium hydroxide solution - acetonitrile (80:20), flow rate 1.5ml/min; temperature 40°C
Authors: Lehr K-H
Source: J Chromatogr 425 (1988) 153 - 161


Details


Legend: Fig. 2. Chromatograms of extracts of the bactericidal drug ofloxacin from samples obtained from a volunteer, serum 5h after an oral dose of 400mg of (±) -ofloxacin containing 1.135µg/ml (-)-ofloxaci