105530 Resolution of chiral amines by HPLC of their mixed chelate complexes with Cu(II)-L-proline

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Resolution of chiral amines by HPLC of their mixed chelate complexes with Cu(II)-L-proline

Summary

Type: HPLC
Application #: 105530
Catalog#: 720120.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x150mm
Detection: fluorescence
Conditions: eluent: 17.5% acetonitrile in a buffer containing 5mM L-proline, 2.5mM copper sulphate pentahydrate and 5.0g/l ammonium acetate, pH 7.0 flow rate 2.0ml/min
Authors: Lam S
Source: J Chromatogr 368 (1986) 413 - 422


Details


Legend: Fig. 1. Chiral separation of OPA-NAC derivatives of D,L-tyrosine, D,L-b-phenylserine, D,L-normetanephrine and D,L-phenylpropanolamine.