105400 Influence of mobile phase composition on optical resolution in ligand exchange HPLC of amino acids

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Influence of mobile phase composition on optical resolution in ligand exchange HPLC of amino acids

Summary

Type: HPLC
Application #: 105400
Catalog#: 720081.40 - MN HPLC Column, Nucleosil Chiral-1, 120A, 5um, 4x250mm
Detection: UV 240 nm
Conditions: eluent A: 0.5 mM aequos copper sulphate
eluent B: eluent A + acetonitrile (10:3)
flow rate: 1.2 ml/min
pressure: 95 bar
temperature: 60°C
Authors: LC department
Source: Macherey-Nagel


Details


Legend: A) Chromatogram with eluent A B) Chromatogram with eluent B