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Confirming chiral HPLC separations with stereospecific enzymes
Summary
Type: HPLCApplication #: 105340
Catalog#: 720120.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x150mm
Detection: fluorescence of dansyl derivatives
Conditions: eluent: 5.0mM L-histidine methyl ester, 2.5mM CuSO4·5H2O and 2.0g ammonium acetate, pH 5.5, stepwise gradient formed by blending the buffer with a 45% acetonitrile solution of the same buffer
flow rate 2.0ml/min
Authors: Lam S., et al.
Source: J. Chromatogr. 468 (1989) 359–364
Details
Legend: Fig. 3. Chromatogram of an amino acid mixture before (upper tracing) and after incubation with D-amino acid oxidase. Oxidative deamination of the D-isomers resulted in reduction in size of peaks cor