105330 Stereoselective D- and L-amino acid analysis by HPLC using mixed chelation with Cu(II)

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Stereoselective D- and L-amino acid analysis by HPLC using mixed chelation with Cu(II)

Summary

Type: HPLC
Application #: 105330
Detection: Fig. 1: fluorescence Fig. 2: fluorescence Fig. 13: -
Conditions: Fig. 1: eluent: buffer A, 5.0mM L-histidine methyl ester, 2.5mM copper sulphate and 2.0g ammonium acetate pH 5.5, buffer B, 45% acetonitrile in buffer A, stepwise gradient Fig. 2: eluent: 5% acetonit
Authors: Lam S
Source: J Chromatogr 289 (1984) 339 - 345


Details


Legend: Fig. 1. from 1. Separation of D,L-amino acid standards as Dns derivatives. Fig. 2. from 2. Chiral separation of OPA derivatives of amino acids. Fig. 13. from 3. Separation of stereoisomers of prolin