105320 Enantiomeric analysis of a mixture of the common protein amino acids as their Dns derivatives. Single-analysis reversed phase HPLC procedure usin

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Enantiomeric analysis of a mixture of the common protein amino acids as their Dns derivatives. Single-analysis reversed phase HPLC procedure using a chiral mobile phase additive

Summary

Type: HPLC
Application #: 105320
Catalog#: 720014.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x250mm
Detection: fluorescence detection: excitation 340nm, emission 425nm
Conditions: gradient program in 20min from 23.5 to 25.1% (v/v) MeCN, in 10min to 26.8% MeCN, in 10min to 28.5% MeCN, in 10min to 31.7% MeCN, in 10min to 35.6% MeCN, in 5min to 40.0% MeCN
flow rate 0.8ml/min
Authors: Weinstein S
Source: J Chromatogr 303 (1984) 244 - 250


Details


Legend: Fig. 1. Separation of almost all amino acids present in a protein hydrolyzate analyzed as their fluorescent Dns derivatives. * indicate derivatization side-products (7min: Dns-OH, 45µin: Dns-amide),