105300 Separation of the xanthine stimulants caffeine theophylline and theobromine

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Separation of the xanthine stimulants caffeine theophylline and theobromine

Summary

Type: HPLC
Application #: 105300
Catalog#: 720990.46 - MN HPLC Column, Nucleosil C8, 100A, 5um, 4.6x150mm
Detection: UV, 280nm
Conditions: eluent: methanol + 0.01M phosphate buffer pH 7 (20 + 80, v/v)
flow rate 1.2ml/min
pressure 130bar
temperature 25°C
Authors: Kipp Analytica
Source: Kipp Analytica Delft The Netherlands 1978


Details


Legend: Separation of 1. theobromine, 2. theophylline and 3. caffeine, injection volume 20µl.