105180 Simple HPLC method for rapid determination of nicotine and cotinine in urine

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Simple HPLC method for rapid determination of nicotine and cotinine in urine

Summary

Type: HPLC
Application #: 105180
Detection: UV, 260nm
Conditions: eluent: dichloromethane - diisopropyl ether - methanol - 25% ammonia (62:30:7.9:0.1)
flow rate 2.0ml/min
Authors: Horstmann M
Source: J Chromatogr 344 (1985) 391 - 396


Details


Legend: Fig. 1. Chromatograms of extracts of A) smoker's urine, B) nonsmokers urine (blank urine, exceptionally not spiked with internal standard) and C) blank urine spiked with 1µg/ml nicotine and 1µg/ml c