105140 Simultaneous determination of nefiracetam and its metabolites by HPLC

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Simultaneous determination of nefiracetam and its metabolites by HPLC

Summary

Type: HPLC
Application #: 105140
Catalog#: 720014.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x250mm
Detection: UV, 210nm
Conditions: eluent A: acetonitrile - 1/60M KH2PO4 pH 4.7 (1:9, v/v), eluent B: acetonitrile - water (50:50), gradient 12min isocratic 100% A, then 0 to 40% B in 18min
flow rate 1.0ml/min
Authors: Fujimaki Y
Source: J Chromatogr 575 (1992) 261 - 268


Details


Legend: Fig. 1. Chromatograms of A) blank human urine, B) human urine spiked with 17.2 nmol/ml nefiracetam (I), 34.0 nmol/ml 5-hydroxynefiracetam (II), 32.6 nmol/ml 4-hydroxynefiracetam (III) and 38.0 nmol/ml