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Determination of N-acetyl-L-cysteine in biological fluids
Summary
Type: HPLCApplication #: 105130
Catalog#: 720110.46 - MN HPLC Column, Nucleosil C18, 100A, 7um, 4.6x150mm
Detection: UV-VIS, 470nm
Conditions: eluent: aqueous 0.5% Na2HPO4 - acetonitrile (70:30, v/v)
flow rate 1.6ml/min
Authors: Frank H
Source: J Chromatogr 309 (1984) 261 - 267
Details
Legend: Fig. 1. HPLC of N-acetyl-S-NBD-cysteine. Left: aqueous solution of N-acetylcysteine, 153µmol/l; right: urine containing 140µmol/l endogenous N-acetylcysteine.