104850 Automated HPLC technique for determining diltiazem and its three main metabolites in serum

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Automated HPLC technique for determining diltiazem and its three main metabolites in serum

Summary

Type: HPLC
Application #: 104850
Catalog#: 720014.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x250mm
Detection: UV, 240nm
Conditions: eluent: 0.005M heptanesulphonic acid - acetonitrile - methanol - glacial acetic acid - triethylamine (60:35:5:5.5:0.75) flow rate 1.5ml/min
Authors: Leneveu A
Source: J Liquid Chromatogr 14 (1991) 3519 - 3530


Details


Legend: Fig. 1. Chromatograms of (C.1) blank serum, (C.2) human standard spiked with A) 20ng/ml M2 B) 20ng/ml M1 C) 20ng/ml MA D) 100ng/ml diltiazem (C.3) sample from a volunteer after a single 120mg o