104820 Determination of trace amounts of 4-aminomorpholine in molsidomine and linsidomine

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Determination of trace amounts of 4-aminomorpholine in molsidomine and linsidomine

Summary

Type: HPLC
Application #: 104820
Detection: UV 235 nm
Conditions: eluent A: 50 mM potassium dihydrogen phosphate, eluent B: methanol, eluent C: acetonitrile - water (80 : 20, v/v). The initial eluent was A - B - C (80:20:0, v/v/v). After injection a stepwise gradient was applied as follows: 5 min 80% A - 20% B - 0% C, then in 25 min to 80% A - 20% B - 0% C, then in 5 min to 0% A - 0% B - 100% C, finally back to starting conditions (80% A - 20% B - 0% C) in 5 min
flow rate 0.5 ml/min
Authors: Körner A
Source: J Chromatogr A 689 (1995) 235 - 245


Details


Legend: Fig. 3. Chromatograms demonstrating the selectivity of precolumn derivatization for molsidomine. Overlay of a chromatogram obtained with a standard solution containing 0.1 % of 4-aminomorpholine and