104720 Plasma level monitoring of D,L-verapamil and three of its metabolites by reversed phase HPLC

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Plasma level monitoring of D,L-verapamil and three of its metabolites by reversed phase HPLC

Summary

Type: HPLC
Application #: 104720
Catalog#: 720002.40 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4x125mm
Detection: fluorescence, excitation 280nm, emission 313nm
Conditions: eluent A for 2.1 and 2.2: acetonitrile - 0.07% o-phosphoric acid (33:67, v/v), eluent B for 2.3 and 2.4: acetonitrile - 0.07% o-phosphoric acid (25:75, v/v)
flow rate 1.5ml/min
Authors: Köppel C
Source: J Chromatogr 570 (1991) 229 - 234


Details


Legend: Fig. 2. HPLC profiles of 2.1) standard solution of 50ng verapamil (V) and 50ng/ml norverapamil (M1), 2.2) plasma sample from a patient, 2.3) standard solution of 50ng/ml M2 and 50ng/ml M3, 2.4) plas