104650 Normal phase HPLC method for the determination of diprafenone in plasma

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Normal phase HPLC method for the determination of diprafenone in plasma

Summary

Type: HPLC
Application #: 104650
Detection: UV, 250nm
Conditions: eluent: methanol - hexane - dist. water - ammonium hydroxide (100:40:10:0.1, v/v)
flow rate 1.5ml/min
Authors: Austin D
Source: J Chromatogr 527 (1990) 182 - 188


Details


Legend: Fig. 2. Chromatogram of plasma spiked with internal standard (A, 500ng/ml), diprafenone (B, 400ng/ml), and 5-hydroxydiprafenone (C, 400ng/ml), injection volume 75µl. Fig. 3. Chromatogram of a pla