104530 HPLC determination of nitrazepam and its metabolites in human urine

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HPLC determination of nitrazepam and its metabolites in human urine

Summary

Type: HPLC
Application #: 104530
Catalog#: 720120.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x150mm
Detection: UV, 254nm
Conditions: eluent: methanol - water (35:65) adjusted to pH 4.0 with phosphoric acid
flow rate 1.3ml/min
column temperature 50°C
pressure 120kg/cm2
Authors: Kozu T
Source: J Chromatogr 310 (1984) 213 - 218


Details


Legend: Fig. 2. Typical chromatogram of nitrazepam and metabolites, 1. 7-aminonitrazepam, 2. 7-acetamidonitrazepam, 3. nitrazepam, 4. nimetazepam (int. std.). Fig. 3. Chromatogram of the extract of human u