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Summary
Type: HPLCApplication #: 104460
Catalog#: 720023.40 - MN HPLC Column, Nucleosil C18, 100A, 10um, 4x250mm
Detection: UV-VIS, 655nm
Conditions: eluent for precolumn enrichment: methanol - water (5:95, v/v), flow rate 1.3ml/min, 10min
eluent for backflush and analytical separation: 1.6M ammonium formate buffer pH 4.0 (2.5% formic acid) - acetonitrile - water (50:20:30, v/v), flow rate 1.3ml/min
Authors: Payet B
Source: J Chromatogr 424 (1988) 337 - 345
Details
Legend: Fig. 3. Typical chromatograms of urine (top) and plasma (bottom) samples, A) control urine (500µl) and plasma (300µl), B) control urine and plasma spiked with known amounts of mitoxantrone, M1 and M