104230 HPLC determination of ibuprofen and metabolites in biological fluids

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HPLC determination of ibuprofen and metabolites in biological fluids

Summary

Type: HPLC
Application #: 104230
Catalog#: 720013.46 - MN HPLC Column, Nucleosil C8, 100A, 5um, 4.6x250mm
Detection: UV, 220nm
Conditions: eluent for assay I: methanol - water (65:35, v/v) with 1 ml/l conc. phosphoric acid
eluent for assay II: 270ml acetonitrile, 730ml water, 1ml conc. phosphoric acid
flow rate 1.6ml/min
Authors: Geisslinger G
Source: J Chromatogr 491 (1989) 139 - 149


Details


Legend: Fig. 1. Chromatograms of ibuprofen and its major metabolites, A) blank human plasma (assay I) C) blank human urine (assay I), D) human plasma spiked with 10µg/ml ibuprofen racemate, (assay I), E) hu