104210 Effects of system peaks in a column-switching liquid chromatographic system for bioanalysis

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Effects of system peaks in a column-switching liquid chromatographic system for bioanalysis

Summary

Type: HPLC
Application #: 104210
Catalog#: 720141.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x100mm
Detection: UV, 254nm
Conditions: precolumn injection procedure: 1ml water, 1ml methanol, 1ml water, 22μl sample, 50μl water

eluents: upper chromatogram, phosphate buffer pH 6.1 - methanol p.a. (1:1)
lower chromatogram, phosphate buffer pH 5.9 - methanol, chromatography grade (1:1)
flow rate 0.89ml/min
Authors: Arvidsson, T.
Source: J. Chromatogr. 407 (1987) 49–58


Details


Legend: Fig. 1. System peaks (A and B) from mobile phase impurities. Sample: 1. oxyphenbutazone (0.94µg/ml); 2. g-hydroxyphenylbutazone (1.19µg/ml), 3. phenylbutazone (1.03µg/ml), in equine blank plasma.