104030 Separation of 4 different forms of CGA derived peptides in a peptid digest.

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Separation of 4 different forms of CGA derived peptides in a peptid digest.

Summary

Type: HPLC
Application #: 104030
Catalog#: 720041.40 - MN HPLC Column, Nucleosil C18, 120A, 5um, 4x250mm
Detection: A : UV 214 nm C : MALDI-TOF von Peaks 1-4
Conditions: Solvent A : 0.05% Trifluoroacatic acid
Solvent B : Acetonitrile containing 0.05% trifluoroacatic acid
Gradient : Start 0% B, after 10 min 25% B; after 60 min 75 % B
Authors: Metz-Boutigue MH
Source: J of biologigal chemistry


Details


Legend: Glycolysated and phosphorylated CGA-derived fragment 173-194 (3055 m/e) with addition of one oxygen on a sugar group (3070 m/e) and with loss of neuranimic acid (2763 m/e) Glycolysated CGA-derived