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Summary
Type: HPLCApplication #: 103920
Catalog#: 720090.46 - MN HPLC Column, Nucleosil CN, 100A, 5um, 4.6x250mm
Detection: UV, 214nm or 240nm for low concentrations of amitriptyline
Conditions: eluent: acetonitrile - methanol -0.01M K2HPO4 buffer, adjusted to pH 6.8 with 85% phosphoric acid (578:188:235, v/v)
flow rate 1.5ml/min
Authors: Härtter S
Source: J Chromatogr 578 (1992) 273 - 282
Details
Legend: Fig. 4. Chromatogram of a plasma sample obtained from a patient who had received daily oral doses of 150 mg amitriptyline for at least seven days, 1. amitriptyline, 2. nortriptyline, 3. E-10-hydroxy