103830 Determination of anticonvulsant drugs and methyl xanthine derivatives in serum
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Determination of anticonvulsant drugs and methyl xanthine derivatives in serum
Summary
Type: HPLCApplication #: 103830
Catalog#: 720120.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x150mm
Detection: UV 230nm
Conditions: eluent A for precolumn loading and washing: 14mM NaH2PO4 - 6mM Na2HPO4,
eluent B for precolumn backflush and analytical separation: eluent A - acetonitrile - methanol (6.5:1.5:2, v/v),
flow rate 0.8ml/min; detection: UV, 230nm
Authors: Haginaka, J. et al.
Source: J. Chromatogr. 529 (1990) 455–461
Details
Legend: Chromatogram after pretreatment of serum with the home-made ISPR precolumn, injection volume 20µl. primidone (10µg/ml), phenobarbital (10µg/ml, phenytoin (20µg/ml), carbamazepine (5µg/ml).