103740 HPLC analysis of polymyxins B and E

Contact an Account Manager

HPLC analysis of polymyxins B and E

Summary

Type: HPLC
Application #: 103740
Catalog#: 720120.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x150mm
Detection: UV, 220nm
Conditions: Fig. 1: ; eluent: 22% acetonitrile in 0.023M H3PO4, 0.01M acetic acid, 0.05M Na2SO4 buffer adjusted to pH 2.5 with triethylamine
Fig. 3: eluent: 23% acetonitrile

flow rate 0.9ml/min
Authors: Elverdam I
Source: J Chromatogr 218 (1981) 653 - 661


Details


Legend: Fig. 1. Chromatogram of 100µg colistin sulphate. Fig. 3. Chromatogram of 100µg polymyxin B sulphate