103730 Separation of polymyxins and octapeptins by HPLC

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Separation of polymyxins and octapeptins by HPLC

Summary

Type: HPLC
Application #: 103730
Catalog#: 720014.40 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4x250mm
Detection: UV, 220nm
Conditions: Fig. 2: eluent: A) 21%, B) 22.5%, C) 30% acetonitrile in 0.005M tartrate buffer, 0.005M Na 1-butanesulphonate, 0.05M Na2SO4
Fig. 3: eluent: A) 30%, B) 31% acetonitrile in buffer as in Fig. 2.
flow rate 1ml/min
Authors: Terabe, S. et al.
Source: J. Chromatogr. 173 (1979) 313–320


Details


Legend: Fig. 2. Separation of A) polymyxins M, D, C and S, B) polymyxins C, E, S and B, C) polymyxins F and T. Fig. 3. Chromatograms of A) 5.2µg octapeptin C1 and B) 250µg EM49 (a mixture of octapeptin A a